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Essentially the most economical ligation temperature for blunt-ended DNA would hence be the temperature at which the greatest variety of alignments can manifest. Nearly all of blunt-finished ligations are performed at fourteen-twenty five °C overnight. The absence of stably annealed ends also means that the ligation performance is decreased, necessitating a greater ligase concentration for use.[fourteen]
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Despite the fact more info that DNA self-assembly is feasible with no outside the house help utilizing distinct substrates like provision of catatonic area of Aluminium foil, DNA ligase can offer the enzymatic help that is needed to make DNA lattice construction from DNA about hangs.[fifteen] History[edit]
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